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tunel detection solution  (Beyotime)


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    Beyotime tunel detection solution
    Tunel Detection Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2049 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tunel+staining+solution/DAPI+Staining+Solution/pmc12960656-441-14-17
    Average 99 stars, based on 2049 article reviews
    tunel detection solution - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    TUNEL Assay:

    Article Title: Targeting PGAM5 attenuates airway inflammation in asthma by inhibiting HMGB1 release in bronchial epithelium.
    Article Snippet: Previous studies have demonstrated that high-mobility group box protein 1(HMGB1) was increased and released to the extracellular and participated in the pathogenesis of steroid-insensitive asthma induced by toluene diisocyanate (TDI).. Mitochondrial dysfunction of bronchial epithelia is a critical feature in TDI asthma.. However, whether mitochondrial dysfunction regulated HMGB1 release in asthma remains unknown.

    Article Title: YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53.
    Article Snippet: For H9c2 cell apoptosis determination, the cells were cultured on the glass slide, fixed with 4% paraformaldehyde (Beyotime, China), and treated with 0.1% Trition X-100 (Beyotime, China) for 2 min on the ice. .. Next, these cells were incubated with TUNEL staining solution (Beyotime, China) for 1 h in the dark. ..

    Article Title: PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway.
    Article Snippet: .. Sections were washed with PBS, stained with 50 μL of TUNEL staining solution at 37 °C for 60 min, rinsed with PBS, stained with DAPI staining solution (C1005, Beyotime) at room temperature for 5 min, and protected from light. ..

    Article Title: Triptolide reverses cis‑diamminedichloroplatinum resistance in esophageal squamous cell carcinoma by suppressing glycolysis and causing mitochondrial malfunction
    Article Snippet: .. The sections were then rinsed with PBS and stained with 50 μl TUNEL staining solution (cat. no. C1005; Beyotime Institute of Biotechnology) at 37°C for 60 min, before being washed with PBS and incubated with DAPI staining solution (cat. no. C1005; Beyotime Institute of Biotechnology) at room temperature in the dark for 5 min. ..

    Article Title: PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway
    Article Snippet: .. Sections were washed with PBS, stained with 50 μL of TUNEL staining solution at 37 ∘ C for 60 min, rinsed with PBS, stained with DAPI staining solution (C1005, Beyotime) at room temperature for 5 min, and protected from light. ..

    Article Title: Effect of ibuprofen on the behavior, histopathology, antioxidant system, DNA damage, and apoptosis of Asian freshwater clams (Corbicula fluminea).
    Article Snippet: The accumulation of ibuprofen (IBU) in freshwater sediments poses significant risks to benthic bivalves, yet its chronic ecological impacts remain insufficiently studied.. Here, Asian clams (Corbicula fluminea) were exposed to 0, 20, 200, and 2000 μg/L IBU for 28 days.. The viscera were identified as the primary target organ for IBU toxicity, with bioconcentration factors (BCFs) ranging from 3.03–6.05 across treatments and a 1.3to 3.3-fold increase in in digestive gland vacuolation damage.

    Article Title: YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53.
    Article Snippet: .. Subsequently, the tissue sections were incubated with the TUNEL staining solution (Beyotime, China) for 2 h at room temperature. .. Finally, the sections were observed and photographed under a fluorescence microscope (Lecia, Germany).

    Article Title: Gen inhibiting the Wnt/Ca 2+ signaling pathway alleviates cerebral ischemia/reperfusion injury.
    Article Snippet: .. The sections were allowed to reach room temperature, after which 4% paraformaldehyde was applied for fixation for 30 to 60 min. After two PBS washes (10 min each), the sections were incubated with 0.5% Triton X-100 at room temperature for 5 min. After two PBS washes, the sections were treated with TUNEL staining solution (Beyotime) and incubated in the dark at 37 °C for 60 min. ..

    Staining:

    Article Title: Targeting PGAM5 attenuates airway inflammation in asthma by inhibiting HMGB1 release in bronchial epithelium.
    Article Snippet: Previous studies have demonstrated that high-mobility group box protein 1(HMGB1) was increased and released to the extracellular and participated in the pathogenesis of steroid-insensitive asthma induced by toluene diisocyanate (TDI).. Mitochondrial dysfunction of bronchial epithelia is a critical feature in TDI asthma.. However, whether mitochondrial dysfunction regulated HMGB1 release in asthma remains unknown.

    Article Title: YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53.
    Article Snippet: For H9c2 cell apoptosis determination, the cells were cultured on the glass slide, fixed with 4% paraformaldehyde (Beyotime, China), and treated with 0.1% Trition X-100 (Beyotime, China) for 2 min on the ice. .. Next, these cells were incubated with TUNEL staining solution (Beyotime, China) for 1 h in the dark. ..

    Article Title: PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway.
    Article Snippet: .. Sections were washed with PBS, stained with 50 μL of TUNEL staining solution at 37 °C for 60 min, rinsed with PBS, stained with DAPI staining solution (C1005, Beyotime) at room temperature for 5 min, and protected from light. ..

    Article Title: Triptolide reverses cis‑diamminedichloroplatinum resistance in esophageal squamous cell carcinoma by suppressing glycolysis and causing mitochondrial malfunction
    Article Snippet: .. The sections were then rinsed with PBS and stained with 50 μl TUNEL staining solution (cat. no. C1005; Beyotime Institute of Biotechnology) at 37°C for 60 min, before being washed with PBS and incubated with DAPI staining solution (cat. no. C1005; Beyotime Institute of Biotechnology) at room temperature in the dark for 5 min. ..

    Article Title: PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway
    Article Snippet: .. Sections were washed with PBS, stained with 50 μL of TUNEL staining solution at 37 ∘ C for 60 min, rinsed with PBS, stained with DAPI staining solution (C1005, Beyotime) at room temperature for 5 min, and protected from light. ..

    Article Title: Effect of ibuprofen on the behavior, histopathology, antioxidant system, DNA damage, and apoptosis of Asian freshwater clams (Corbicula fluminea).
    Article Snippet: The accumulation of ibuprofen (IBU) in freshwater sediments poses significant risks to benthic bivalves, yet its chronic ecological impacts remain insufficiently studied.. Here, Asian clams (Corbicula fluminea) were exposed to 0, 20, 200, and 2000 μg/L IBU for 28 days.. The viscera were identified as the primary target organ for IBU toxicity, with bioconcentration factors (BCFs) ranging from 3.03–6.05 across treatments and a 1.3to 3.3-fold increase in in digestive gland vacuolation damage.

    Article Title: YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53.
    Article Snippet: .. Subsequently, the tissue sections were incubated with the TUNEL staining solution (Beyotime, China) for 2 h at room temperature. .. Finally, the sections were observed and photographed under a fluorescence microscope (Lecia, Germany).

    Article Title: Gen inhibiting the Wnt/Ca 2+ signaling pathway alleviates cerebral ischemia/reperfusion injury.
    Article Snippet: .. The sections were allowed to reach room temperature, after which 4% paraformaldehyde was applied for fixation for 30 to 60 min. After two PBS washes (10 min each), the sections were incubated with 0.5% Triton X-100 at room temperature for 5 min. After two PBS washes, the sections were treated with TUNEL staining solution (Beyotime) and incubated in the dark at 37 °C for 60 min. ..

    Incubation:

    Article Title: Targeting PGAM5 attenuates airway inflammation in asthma by inhibiting HMGB1 release in bronchial epithelium.
    Article Snippet: Previous studies have demonstrated that high-mobility group box protein 1(HMGB1) was increased and released to the extracellular and participated in the pathogenesis of steroid-insensitive asthma induced by toluene diisocyanate (TDI).. Mitochondrial dysfunction of bronchial epithelia is a critical feature in TDI asthma.. However, whether mitochondrial dysfunction regulated HMGB1 release in asthma remains unknown.

    Article Title: YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53.
    Article Snippet: For H9c2 cell apoptosis determination, the cells were cultured on the glass slide, fixed with 4% paraformaldehyde (Beyotime, China), and treated with 0.1% Trition X-100 (Beyotime, China) for 2 min on the ice. .. Next, these cells were incubated with TUNEL staining solution (Beyotime, China) for 1 h in the dark. ..

    Article Title: Triptolide reverses cis‑diamminedichloroplatinum resistance in esophageal squamous cell carcinoma by suppressing glycolysis and causing mitochondrial malfunction
    Article Snippet: .. The sections were then rinsed with PBS and stained with 50 μl TUNEL staining solution (cat. no. C1005; Beyotime Institute of Biotechnology) at 37°C for 60 min, before being washed with PBS and incubated with DAPI staining solution (cat. no. C1005; Beyotime Institute of Biotechnology) at room temperature in the dark for 5 min. ..

    Article Title: Effect of ibuprofen on the behavior, histopathology, antioxidant system, DNA damage, and apoptosis of Asian freshwater clams (Corbicula fluminea).
    Article Snippet: The accumulation of ibuprofen (IBU) in freshwater sediments poses significant risks to benthic bivalves, yet its chronic ecological impacts remain insufficiently studied.. Here, Asian clams (Corbicula fluminea) were exposed to 0, 20, 200, and 2000 μg/L IBU for 28 days.. The viscera were identified as the primary target organ for IBU toxicity, with bioconcentration factors (BCFs) ranging from 3.03–6.05 across treatments and a 1.3to 3.3-fold increase in in digestive gland vacuolation damage.

    Article Title: YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53.
    Article Snippet: .. Subsequently, the tissue sections were incubated with the TUNEL staining solution (Beyotime, China) for 2 h at room temperature. .. Finally, the sections were observed and photographed under a fluorescence microscope (Lecia, Germany).

    Article Title: Gen inhibiting the Wnt/Ca 2+ signaling pathway alleviates cerebral ischemia/reperfusion injury.
    Article Snippet: .. The sections were allowed to reach room temperature, after which 4% paraformaldehyde was applied for fixation for 30 to 60 min. After two PBS washes (10 min each), the sections were incubated with 0.5% Triton X-100 at room temperature for 5 min. After two PBS washes, the sections were treated with TUNEL staining solution (Beyotime) and incubated in the dark at 37 °C for 60 min. ..

    Microscopy:

    Article Title: Targeting PGAM5 attenuates airway inflammation in asthma by inhibiting HMGB1 release in bronchial epithelium.
    Article Snippet: Previous studies have demonstrated that high-mobility group box protein 1(HMGB1) was increased and released to the extracellular and participated in the pathogenesis of steroid-insensitive asthma induced by toluene diisocyanate (TDI).. Mitochondrial dysfunction of bronchial epithelia is a critical feature in TDI asthma.. However, whether mitochondrial dysfunction regulated HMGB1 release in asthma remains unknown.



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    Image Search Results


    a Representative HE-staining images of the upper duodenum in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 400 μm. b Representative HE-staining images of the ileum in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 200 μm. c TUNEL staining images of the ileum in mice. Scale bar: 400 μm. d Statistical analysis of ileum goblet cells per villus across different groups. e Statistical analysis of colon goblet cells per crypt across different groups. f Statistical analysis of ( c ). g Representative HE-staining images of the colon in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 200 μm. h TUNEL staining images of the colon in mice. Scale bar: 200 μm. One-way ANOVA test was used between multiple comparisons, and unpaired two-tailed Student’s t -test was applied for comparison between two groups.

    Journal: NPJ Parkinson's Disease

    Article Title: Dysfunctional digestive tract highlights the metabolic hallmarks of nanoplastic-exacerbated Parkinson’s pathology

    doi: 10.1038/s41531-025-01145-2

    Figure Lengend Snippet: a Representative HE-staining images of the upper duodenum in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 400 μm. b Representative HE-staining images of the ileum in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 200 μm. c TUNEL staining images of the ileum in mice. Scale bar: 400 μm. d Statistical analysis of ileum goblet cells per villus across different groups. e Statistical analysis of colon goblet cells per crypt across different groups. f Statistical analysis of ( c ). g Representative HE-staining images of the colon in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 200 μm. h TUNEL staining images of the colon in mice. Scale bar: 200 μm. One-way ANOVA test was used between multiple comparisons, and unpaired two-tailed Student’s t -test was applied for comparison between two groups.

    Article Snippet: Each section was covered with 50 μL TUNEL staining solution (Yeasen, China) and incubated at 37 °C in darkness for 1 h, followed by 3 PBS washes.

    Techniques: Staining, Injection, TUNEL Assay, Two Tailed Test, Comparison